Explicit simulations of ionic conductance through the transmembrane domain of MscS. The pore occupancy with ions in the course of two 16-ns simulations at +100 mV (A) and −100 mV (B). The occupancies were measured for the region of the pore (−12 > z > −32 Å) encompassing the gate. Panel C shows the cumulative charge transferred by ions passing through the gate region during these two simulations. The direction of ion movement is denoted by the traces. Integrals of ion and water passage events for two 8-ns simulations at +200 and −200 mV (D). The scale for the number of permeated water molecules is on the right and reflects ∼8 waters per ion. The bulk concentration of KCl is 1.5 M.