Effect of low K⁺ and Cl⁻ concentrations on growth of sul1, gef1, and vma1 strains of S. cerevisiae. Cells from each strain were suspended at initial A₅₉₀ of 0.1 and incubated aerobically at 30°C with rotary shaking for 24 h. The medium was derived from LCAPG, with increasing amounts of Cl⁻ added as arginine Cl⁻. The initial concentrations of Cl⁻ in the media were ∼0.005, 0.035, 0.10, 0.30, 1.0, and 3.0 mM (left to right) in each group of six bars. The initial concentrations of K⁺ (added as KH₂PO₄) were 0.1, 0.3, 1.0, and 3.0 mM.