Figure 7.
VRAC in peritoneal macrophages from wild-type and mBest1−/−-mBest2−/− mice. (A, C, and E) Wild-type mice. (B, D, and F) mBest1−/−-mBest2−/− mice. The intracellular solution of all recordings was held constant at 306 mosmol kg−1, whereas the osmolality of the extracellular solution was altered to achieve different osmotic pressures. (A and B) Current traces under isosmotic conditions. (C and D) Current traces in Δ40 mosmol kg−1 hyposmotic conditions (266 mosmol kg−1 extracellular). (E and F) Average current–voltage relationships under Δ70 mosmol kg−1 hyposmotic (open symbols; 234 mosmol kg−1extracellular) and −Δ20 mosmol kg−1 hyperosmotic (filled symbols; 326 mosmol kg−1 extracellular) conditions.
