Effect of acute salt repletion on β and γENaC expression in total membranes and in biotin-neutravidin eluates. Each lane was loaded with 40 μg total membrane protein or 20 μl eluate. Staining of the major band of βENaC (top) and the low-molecular mass peptide of γENaC (bottom) were decreased after 5 h salt repletion.