Effects of aldosterone infusion on β and γENaC expression in total membranes and in biotin-neutravidin eluates. Each lane was loaded with 45 μg total membrane protein or 20 μl eluate. Top: staining of the major band of βENaC was slightly decreased in the total membranes but increased in the surface pool of aldosterone-treated animals. Bottom: staining of the lower molecular mass form of γENaC was increased in both the total membranes and in the surface pool.