Figure 4.
Figure 4. Effect of α-ENaC K149A/R164A/K169A/R177A mutant on CAP2-induced INa and α-ENaC N-terminal fragments. (A) Western blots of α-ENaC N-terminal 17- and 19-kD novel fragments at the surface (top) and total pools (bottom). Lane 1, uninjected eggs; lane 2, ENaC alone; lane 3, ENaC plus CAP2; lane 4, α-ENaC mutant alone; lane 5, α-ENaC mutant plus CAP2. FL, full-length. A representative experiment is shown (n = 3). (B) INa of WT and mutant α-ENaC stimulated by CAP2. Batches of oocytes were extracted from three different frogs (n = 18). * and **, P < 0.0001. Amiloride-sensitive currents were measured as described in Fig. 1.

Effect of α-ENaC K149A/R164A/K169A/R177A mutant on CAP2-induced INa and α-ENaC N-terminal fragments. (A) Western blots of α-ENaC N-terminal 17- and 19-kD novel fragments at the surface (top) and total pools (bottom). Lane 1, uninjected eggs; lane 2, ENaC alone; lane 3, ENaC plus CAP2; lane 4, α-ENaC mutant alone; lane 5, α-ENaC mutant plus CAP2. FL, full-length. A representative experiment is shown (n = 3). (B) INa of WT and mutant α-ENaC stimulated by CAP2. Batches of oocytes were extracted from three different frogs (n = 18). * and **, P < 0.0001. Amiloride-sensitive currents were measured as described in Fig. 1.

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