Agonist-induced biphasic current response is independent of pannexin-1 channels. (A) Agonist-induced diffusion of extracellular YO-PRO-1 into GT1 cells. Fluorescence intensities expressed in arbitrary units (λex = 488 nm) are shown in response to application of BzATP only (left trace) and ATP followed by BzATP (right trace). (B) Normalized fluorescence intensities (gray, λex = 380 nm; black, λex = 340 nm) in GT1 cells loaded with Fura-FF (top) or Fura-2 (bottom) and stimulated with 100 μM BzATP. (C and D) BzATP-induced biphasic response in GT1 (C) and C6 single cells (D). (E) The lack of effects of CBX on pattern of current response in HEK293 cells; control (left) and 30 μM CBX-treated cells (right). In experiments shown in A, C, D, and E, cells were bathed in KR buffer. In experiments shown in B, cells were bathed in Ca2+-deficient KR buffer.
