Figure 2.
Figure 2. ECFP-CaM overexpression in HEK293 cells. (A) Epifluorescence image and intensity profile of an HEK293 cell expressing ECFP-CaM alone shows uniform cytosolic expression of CaM. (B) Immunoblot of HEK293 lysates probed with an anti-CaM antibody demonstrates endogenous CaM, ECFP-CaM, and ECFP-CaM1234 (left). Immunoreactive ECFP, ECFP-CaM, and ECFP-CaM1234 are shown with an anti-GFP antibody. (C) Epifluoresecence image and intensity profile of NaV1.4-EYFP coexpressed with ECFP-CaM in HEK293 cells; the peaks indicate membrane targeting. In the top panel, the cell is excited with 535-nm light and the bottom panel is the same cell excited with 440-nm light showing enrichment of ECFP-CaM at the cell membrane when coexpressed with NaV1.4-EYFP.

ECFP-CaM overexpression in HEK293 cells. (A) Epifluorescence image and intensity profile of an HEK293 cell expressing ECFP-CaM alone shows uniform cytosolic expression of CaM. (B) Immunoblot of HEK293 lysates probed with an anti-CaM antibody demonstrates endogenous CaM, ECFP-CaM, and ECFP-CaM1234 (left). Immunoreactive ECFP, ECFP-CaM, and ECFP-CaM1234 are shown with an anti-GFP antibody. (C) Epifluoresecence image and intensity profile of NaV1.4-EYFP coexpressed with ECFP-CaM in HEK293 cells; the peaks indicate membrane targeting. In the top panel, the cell is excited with 535-nm light and the bottom panel is the same cell excited with 440-nm light showing enrichment of ECFP-CaM at the cell membrane when coexpressed with NaV1.4-EYFP.

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