Effect of gtl-1 and gon-2 loss-of-function mutations on pBoc period and rhythmicity. (A) pBoc cycles in individual wild-type, GTL-1 mutant, GON-2 mutant, and double mutant worms. Different colors and symbols represent different animals. (B) pBoc periods and rhythmicity for wild type and GTL-1 and GON-2 mutant worm strains. Cycle rhythmicity is quantified as coefficient of variance. Values are means ± SEM (n = 6–23). *, P < 0.05; ***, P < 0.001, compared with wild type worms. ^†, P < 0.01, compared with GTL-1 mutant worms. ^††, P < 0.001, compared with GON-2 mutant worms. All worm strains were grown at 25°C.