An amperometry recording of the fusion of a single vesicle. The foot reports the opening of a fusion pore and the spike begins when the fusion pore enters into an expansion phase. The features of feet and spikes analyzed in this study are illustrated. We measured the amplitude and duration of the foot, the peak amplitude, total area, width at half-height, time to rise from 35 to 90% of peak, and time to decay from 90% to within 5×RMS of baseline. The method of computing these various parameters is described in Materials and methods.