Coassembly with S1-WT reduces the basal whole-oocyte current and increases the glibenclamide-sensitive current of S2-ND. (A) Time courses of representative whole-cell currents recorded from oocytes expressing S1-WT (0.5 ng; black trace), S2-ND (1.5 ng; blue trace), and S1-WT+S2-ND (0.5 ng and 1.5 ng, respectively; red trace). Currents were recorded at −80 mV by TEVC (see Materials and methods). 3 mM sodium azide (Az), 340 μM diazoxide (Dzx), 10 μM glibenclamide (Gb), and 3 mM BaCl₂ were applied to the bath solution as indicated by the labeled horizontal bars. Approximately 1-min stationary sections (−//−) were omitted from the S1-WT (black) and S2-ND (blue) traces to align the timing of all drug applications for all three traces. The red dotted line is the sum of the black and blue traces and illustrates the whole-cell current expected for an oocyte coexpressing S1-WT and S2-ND assuming that the two subunits do not coassemble. The inset shows the entire S1-WT current trace at an expanded current scale. (B) Bar chart summary of the mean basal (I_Basal), azide-activated (I_Az), diazoxide-activated (I_Dzx), and glibenclamide-sensitive (I_Gb) current components, as well as of the total currents (I_Total) in oocytes expressing S1-WT, S2-ND, and S1-WT+S2-ND.