Analysis of Creb_osb^−/− mice. (A) Specificity of α1(I) Collagen–Cre-driven deletion of Creb allele in bone. (B) Expression level of the CREB protein in bone marrow–derived osteoblasts. (C) Bone histomorphometric analysis of Creb_osb^−/− mice at 12 wk of age (control, n = 14; Creb_osb^−/−, n = 12). BV/TV, bone volume over tissue volume; Ob.Nb/T.Ar, number of osteoblasts per trabecular area; Oc.S/T.Ar, osteoclast surface per trabecular area. (D) µCT analysis of control (n = 5) and Creb_osb^−/− (n = 5) proximal tibiae at 12 wk of age. (E and F) Expression of various genes in control (n = 7) and Creb_osb^−/− (n = 3) bone at 12 wk of age. (G) Serum CTx levels of control (n = 7) and Creb_osb^−/− (n = 7) mice at 12 wk of age. (H–K) Bone histomorphometric analysis of Creb_osb^−/− mice after leptin ICV infusion at 12 wk of age shown as percentage compared with control mice with vehicle ICV infusion (control mice with vehicle ICV infusion, n = 14; control mice with leptin ICV infusion, n = 12; Creb_osb^−/− mice with vehicle ICV infusion, n = 8; Creb_osb^−/− mice with leptin ICV infusion, n = 10). (L) Bone histomorphometric analysis of 12-wk-old Creb_osb^−/− mice after leptin ICV infusion. All experiments were performed independently at least twice, and representative data are shown. Results are shown as mean ± SEM. Statistical analysis was performed by Student’s t test. For all panels: *, P < 0.05.