The gp38⁺CD31⁺ LN stromal cells responsible for mediating tolerance to Tyr₃₆₉ are not related to melanocytes. Immunofluorescence of a normal LN section (a and c) and epidermal ear sheets (b, d, and e) stained for the expression of LN stromal cell markers CD31 (100×; a and b) and gp38 (100×; c and d). Melanocytes in epidermal ear sheets were visualized by their tyrosinase staining (200×; e). Staining is representative of multiple magnifications and fields from two independent experiments consisting of two separate LNs and ears from three mice. Bars, 200 µm.