Binding analysis of various sialylated PA–glycan conjugates to wild-type compared with siglec mutant B cells. Results are representative of three independent experiments. (A–E) B cells of the indicated genotypes were stained with biotinylated PA conjugated with the following glycans: (A) NeuAcα2-6Galβ1-4GlcNAc, (B) NeuGc, (C) bNeuGc, (D) NeuAcα2-3Galβ1-4GlcNAc, and (E), NeuGcα2-3Galβ1-4GlcNAc. Binding was revealed using fluorescent streptavidin. Background staining by streptavidin alone is shown in gray. (F) Chemical structures of the NeuGc-containing sialosides.