Draining CLNs contain five skin-derived DC subsets. Flow cytometry analysis of the skin-derived migratory DCs (MHCII^high CD11c^{inter-to-high}) present in CLNs from C57BL/6 (B6) mice and B6 (CD45.1)→B6 (CD45.2) BM chimeras. MHCII^high CD11c^{inter-to-high} DCs were analyzed for the expression of CD207 versus CD11b and CD103 expression determined on each of the specified DC subsets. (A) A single CD207⁺ CD11b^−/low DC cluster is present in the CLNs of B6 mice. Unlike DDCs, LCs are radio resistant, and this permits the use of B6 (CD45.1)→B6 (CD45.2) chimeras to split the single CD207⁺ CD11b^−/low DC cluster into host-derived mLCs and into CD103⁺ and CD103⁻ donor-derived mDDCs. Two additional mDDC subsets with a CD207⁺ CD11b⁻ and CD207⁻ CD11b⁺ phenotype can be identified in both C57BL/6 mice and B6 (CD45.1)→B6 (CD45.2) chimeras. (B) Expression of CD103 among the DC subsets found in the dermis of B6 (CD45.1)→B6 (CD45.2) BM chimeras. The percentages of cells found in each of the specified gates are indicated. Data shown are representative of at least 12 chimeric mice corresponding to six independent experiments.