Initial host cell tropism of MVA in the lung. Mice were i.n. infected with 107 IU MVA, and BAL cells were analyzed by flow cytometry. (A) GFP expression of DAPI− BAL cells 7 h after infection with MVA-GFP or MVA-WT. (B) GFP expression of BAL 0–72 h after i.n. infection with MVA-GFP (mean + SD; n = 2–3 mice/time point). (C and D) Expression of CD11c and MHCII on all (C) or GFP+ (D) DAPI− BAL cells 7 h after infection with MVA-GFP. (E) Immunohistology of lung sections 6 h after i.n. application of Cy5-labeled MVA using antibodies and dyes as indicated. (F) As in E, we obtained sections from lungs 30 min after MVA-Cy5 application. Bars, 25 µm. (G–I) FACS analysis of BAL cells 3 d after MVA-Cy5 application using antibodies and virus as indicated. (G and H) Gate on all DAPI− cells. (I) Gate on all DAPI− MVA-Cy5+ cells. The FACS plots and immunohistology shown are representative of three independent experiments, each with two to three mice analyzed per time point. *, P < 0.05; **, P < 0.01.
