Figure 1.
Figure 1. Type 2 ATMs are unchanged in lean Mgl1−/− mice. (A) Analysis of ATMs in lean mice for markers of alternative activation. Epididymal white adipose tissue (EWAT) from lean Mgl1−/− and Mgl1+/+ mice were stained for CD206 and CX3CR1. (B) Quantitation of F4/80+ CD206+ ATMs in lean Mgl1−/− and Mgl1+/+ mice. SVF isolated from EWAT (n = 4) and analyzed by flow cytometry. Total ATM numbers were normalized to tissue mass. (C) ST2 expression in type 2 resident ATMs. EWAT from ND-fed Mgl1−/− and control C57BL/6 mice were stained for ST2 (green) and MGL1 (red). Staining and imaging parameters were identical between samples to demonstrate the specificity of the antibodies for MGL1. (D) ST2 expression in obese mice. Isolectin staining identifies CLSs containing type 1 ATM clusters. Samples from EWAT from HFD-fed C57BL/6 mice. (A, C, and D) Representative images shown from one of at least three independent experiments. Bars, 50 µm.

Type 2 ATMs are unchanged in lean Mgl1−/− mice. (A) Analysis of ATMs in lean mice for markers of alternative activation. Epididymal white adipose tissue (EWAT) from lean Mgl1−/− and Mgl1+/+ mice were stained for CD206 and CX3CR1. (B) Quantitation of F4/80+ CD206+ ATMs in lean Mgl1−/− and Mgl1+/+ mice. SVF isolated from EWAT (n = 4) and analyzed by flow cytometry. Total ATM numbers were normalized to tissue mass. (C) ST2 expression in type 2 resident ATMs. EWAT from ND-fed Mgl1−/− and control C57BL/6 mice were stained for ST2 (green) and MGL1 (red). Staining and imaging parameters were identical between samples to demonstrate the specificity of the antibodies for MGL1. (D) ST2 expression in obese mice. Isolectin staining identifies CLSs containing type 1 ATM clusters. Samples from EWAT from HFD-fed C57BL/6 mice. (A, C, and D) Representative images shown from one of at least three independent experiments. Bars, 50 µm.

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