Figure 4. The L503S polymorphic mutation in type II ROP16 determines its defective Stat3 activation. (A–D) 293T cells were transfected with the APRE-luc plasmid together with the indicated ROP16 expression vectors. Luciferase activities were expressed as fold increases over the background levels shown by lysates prepared from mock-transfected cells. Indicated values are means ± the variation range of duplicates. A list of the chimeric ROP16 expression vectors is shown (B, left). R, RH (pink); M, ME49 (blue). (E) Serum-starved MEFs were infected with an MOI = 10 of the indicated parasites for 18 h. Activation of Stat3 was also determined by Western blot analysis of cell extracts using anti–phospho-Stat3 Tyr 705. Stat3 and actin levels are shown as loading controls. Data are representative of three (A and E) or two (B–D) independent experiments.
Figure 4.

The L503S polymorphic mutation in type II ROP16 determines its defective Stat3 activation. (A–D) 293T cells were transfected with the APRE-luc plasmid together with the indicated ROP16 expression vectors. Luciferase activities were expressed as fold increases over the background levels shown by lysates prepared from mock-transfected cells. Indicated values are means ± the variation range of duplicates. A list of the chimeric ROP16 expression vectors is shown (B, left). R, RH (pink); M, ME49 (blue). (E) Serum-starved MEFs were infected with an MOI = 10 of the indicated parasites for 18 h. Activation of Stat3 was also determined by Western blot analysis of cell extracts using anti–phospho-Stat3 Tyr 705. Stat3 and actin levels are shown as loading controls. Data are representative of three (A and E) or two (B–D) independent experiments.

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