Figure 2. Histology of skin lesions before and after VACV infection. (A) Hematoxylin and eosin–stained skin tissues are shown for normal and eczematous mice before and 7 d after virus infection. Bar, 1 mm. (B) CD4+, CD8+, and Mac-1+ cells were stained by immunohistochemistry and mast cells were stained with toluidine blue. Data represent means and SEM values of cell numbers per high-power field (HPF; n = 8 each group). (C) Immunohistochemical staining of NK cells with anti-Ly49G2 (clone 4D11) mAb. Bar, 0.1 mm. (D) Ly49G2 (4D11)+ NK cells were enumerated with six mice per cohort. Shown are results representative of six independent experiments. Data represent means and SEM. *, P < 0.05; **, P < 0.01; and ***, P < 0.001. NS, not significant.
Figure 2.

Histology of skin lesions before and after VACV infection. (A) Hematoxylin and eosin–stained skin tissues are shown for normal and eczematous mice before and 7 d after virus infection. Bar, 1 mm. (B) CD4+, CD8+, and Mac-1+ cells were stained by immunohistochemistry and mast cells were stained with toluidine blue. Data represent means and SEM values of cell numbers per high-power field (HPF; n = 8 each group). (C) Immunohistochemical staining of NK cells with anti-Ly49G2 (clone 4D11) mAb. Bar, 0.1 mm. (D) Ly49G2 (4D11)+ NK cells were enumerated with six mice per cohort. Shown are results representative of six independent experiments. Data represent means and SEM. *, P < 0.05; **, P < 0.01; and ***, P < 0.001. NS, not significant.

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