Ectopic expression of MOLF/Ei or C57BL/6J IRAK-2 isoforms results in similar effects on TLR2 activity. RAW264.7 macrophages were transduced with a lentiviral construct encoding the MOLF/Ei or C57BL/6J alleles of IRAK-2A (A) or IRAK-2C (B). Cells were stimulated for the indicated times with 2 µg/ml LTA. Total levels of IRAK-2 in unstimulated cells as well as IL-6 and TNF mRNA accumulation were assessed by real-time PCR. Data are depicted as mean ± range of duplicate wells, representative of three independent experiments.