CXP lymphomas harbor clonal JH rearrangements, but do not express IgM or IgL chains. (A) Southern blot analyses of CXP lymphoma genomic DNA samples for rearrangement or deletion of JH loci (representative of at least two separate analyses for each sample). (B) Western blot analyses of CXP lymphoma cellular extracts for expression of μ heavy chain and κ or λ light chains using HRP-conjugated anti-IgM (μ heavy chain specific), anti-κ, or anti-λ antibodies, respectively. (C) Western blot analyses of CXP lymphoma cellular extracts for expression of γ heavy chains using HRP-conjugated anti–mouse IgG antibody. An α-tubulin antibody was used to generate a loading control in both panels. PP indicates extracts from Peyer's patches. Data presented in this figure shows results representative of two or three independent sets of analyses.
