DC-induced CD8 T cell stimulation is DNAM-1 independent. (A) BMDCs express of CD155, which is up-regulated by Toll-like receptor–induced DC maturation. (B) Expression of CD155 on CD11c⁺ DCs migrating from the periphery to CLNs 48 h after ear painting. A gate was applied on SSC/FSC to exclude small lymphocytes. (C) Proliferation of WT and DNAM-1^−/− OT-1 in response to immature BMDCs pulsed with OVA for 18 h (left) or with OVA for 3 h followed by stimulation with CpG ODN1826 for 7 h (right). Proliferation was evaluated by [³H]thymidine incorporation. Results are means ± SEM. (D) Percentages of CD8 T cells producing IFN-γ after in vitro restimulation with EL-4–CD155 cells either pulsed or not pulsed with OVA peptide 257–264. Mice (six per group) were tested 8 d after footpad immunization. Horizontal bars represent means. *, P < 0.05.