![Figure 2. DNAM-1 promotes CD8 T cell proliferation in response to antigen presented by nonprofessional APCs. DNAM-1−/− OT-1 transgenic CD8 T cells proliferate less efficiently than WT OT-1 cells in response to SIINFEKL peptide presented by EL-4 T lymphoma cells expressing mCD155. Proliferation was measured (A) by [3H]thymidine incorporation or (B) by evaluating the increase in SSC and FSC by flow cytometry in blasting cells. Dot plot analysis shows stimulation induced by 10 ng/ml SIINFEKL. One representative experiment out of three is shown. Results in A are means ± SEM.](https://cdn.rupress.org/rup/content_public/journal/jem/205/13/10.1084_jem.20081752/7/jem_20081752_lw_fig2.jpeg?Expires=1767676423&Signature=EYtiCLVIkr4UVYr~JzyudKVqPQEV4RC0ZDZKLllejflwKDB7RYZmyyqUVP9ZTVjSSTi5EaKVcx8oMNp7J617qdXLJiv1HHvHHQbRUprHevoZnVhX56lYV~lHGbIsWLSfDLBZRXD4tYKQqDVUn~~iKXcGl2~HK83tECyOVO~ntc8zxPsIMD8gLxgeT4tD5QDyBgOYwVwGXYfJPIqndOLKFcnVgHz3f4~Azg14ZQ38Lg7DiaYoYgjddHzLa12xvh652D3ZHdjQca5s~Is7b~jKIGX89hgYjEfSA7ml2tBlFQo95s8EyGSocuoGW0Et-9rES6k5q1JADiglLw59R-riiw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 2.
DNAM-1 promotes CD8 T cell proliferation in response to antigen presented by nonprofessional APCs. DNAM-1−/− OT-1 transgenic CD8 T cells proliferate less efficiently than WT OT-1 cells in response to SIINFEKL peptide presented by EL-4 T lymphoma cells expressing mCD155. Proliferation was measured (A) by [3H]thymidine incorporation or (B) by evaluating the increase in SSC and FSC by flow cytometry in blasting cells. Dot plot analysis shows stimulation induced by 10 ng/ml SIINFEKL. One representative experiment out of three is shown. Results in A are means ± SEM.
