Proliferation and cytokine production of P0-specific NOD-POT T cells. (A) Proliferation of CFSE-labeled NOD-POT T cells in the presence of APCs alone (gray histogram) or APCs and the indicated stimulus (black histograms). Histograms are gated on the CD4⁺ population. (B) Proliferation of CFSE-labeled NOD-POT spleen cells in NOD or NOD-B7-2KO recipients (22 wk of age) after 4 d (inguinal lymph nodes are shown). Histograms are gated on the Thy1.1⁺CD4⁺ population. (C) Production of IFN-γ (left) and IL-17 (right) in the culture supernatant of NOD-POT cells activated by the indicated stimulus. (D) Production of IFN-γ and IL-17 after stimulation of spleen cells from the indicated mice with PMA and ionomycin. Dot plots are gated on CD4⁺ T cells. Numbers indicate percentages of cells in the quadrants. Data are representative of two or more experiments.