Phenotypic analysis of POT TCR-Tg mice. (A) Phenotyping of CD4⁺ and CD8⁺ T cells in the thymus (left) and spleen (right) of NOD-POT TCR-Tg mice (top) and transgene-negative littermates (bottom). (B) Phenotyping of CD4⁺ T cells in the spleens of NOD-RAGKO-POT TCR-Tg mice. (C) Foxp3 expression in the spleens of the indicated 12-wk-old mice (histograms are gated on CD4⁺ T cells). Numbers indicate percentages of Foxp3⁺ cells in the CD4 population. (A–C). (D) CD25 and Foxp3 expression among CD4⁺ cells in the spleens of the indicated 19-d-old mice. Percentages ± SD are indicated. Data are representative of two or more experiments.