Figure 2. Myelin P0 is targeted by the autoimmune response in NOD-B7-2KO mice. (A) Immunoblotting of sciatic nerve extracts with serum from individual anti–B7-1–treated NOD-B7-2KO neuropathic mice (lanes 1–9), nonneuropathic NOD mice (lanes 10 and 11), or BALB/c controls (lanes 12 and 13). Data are representative of five to nine independent experiments for each serum. The predominant 25–30-kD reactivity is indicated with an arrow. (B) Nerve-specific CD4+ T cell hybridomas were examined for reactivity to P0 as in Fig. 1 D, using pools of overlapping peptides spanning the P0 protein. Data are representative of two experiments. Arrows indicate reactive clones to a specific pool of peptide. (C) Activation of the 2E7 hybridoma by individual P0 peptides from pools #1 and #4. Data are representative of two experiments.
Figure 2.

Myelin P0 is targeted by the autoimmune response in NOD-B7-2KO mice. (A) Immunoblotting of sciatic nerve extracts with serum from individual anti–B7-1–treated NOD-B7-2KO neuropathic mice (lanes 1–9), nonneuropathic NOD mice (lanes 10 and 11), or BALB/c controls (lanes 12 and 13). Data are representative of five to nine independent experiments for each serum. The predominant 25–30-kD reactivity is indicated with an arrow. (B) Nerve-specific CD4+ T cell hybridomas were examined for reactivity to P0 as in Fig. 1 D, using pools of overlapping peptides spanning the P0 protein. Data are representative of two experiments. Arrows indicate reactive clones to a specific pool of peptide. (C) Activation of the 2E7 hybridoma by individual P0 peptides from pools #1 and #4. Data are representative of two experiments.

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