Generation of peripheral nerve–specific T cell hybridoma. (A and B) CD4⁺ T cells were sorted from the nerves of neuropathic NOD-B7-2KO mice (A) and expanded in vitro (B). Data are representative of 15 sorts and expansions. (C) 2–5 × 10⁶ expanded cells were transferred into NOD-SCID or NOD-RAGKO immunodeficient mice, and recipients were followed for the development of neuropathy (n = 5 mice from two independent experiments). (D) CD4⁺ T cell hybridomas were stimulated with APCs alone (open histogram) or in the presence of the indicated stimulus (shaded histograms), and CD69 up-regulation was assessed. Clone 2E7 is shown. Data are representative of four experiments.