Visualizing aortic DCs using CD11c-EYFP transgenic mice. (A) The presence of EYFP⁺ cells in aortic cell suspension from EYFP transgenic mice. Aortic segments from EYFP transgenic mice (n = 5) were pooled and dissociated by incubation with an enzyme mixture (see Materials and methods) and then subjected to flow cytometric analysis. (B) Experiments were performed as in A, but the aortic CD11c⁺ cells were from nontransgenic mice (n = 3) and compared for expression of CD11c and MHC II to aortic EYFP⁺ cells. (C) Immunohistochemical staining of CD11c and MHC II in aortic sheets. Each experiment was performed at least three times. Bars, 20 µm.