Proliferative potential of neutrophil precursor and progenitor cells. (A) Cell cycle analysis of total bone marrow Gr-1⁺ cells from WT and ARKO mice. Comparison of cell cycle distributions shows that 9-wk-old ARKO mice have a lower percentage of cells in the S-phase and the G2/M phase of the cell cycle compared with WT littermates. Data are presented as the mean ± standard deviations (SD) of three independent experiments in the right panel. (B) Kinetics of BrdU labeled neutrophils in the blood of WT and ARKO mice after BrdU injection. (C) Comparison of BrdU incorporation in WT and ARKO blood neutrophils. At 96 h post injection, the ratio of BrdU^dim in blood neutrophils is lower in 9-wk-old AR-deficient mice (n = 4) as compared with WT littermates (n = 4). For each group, 10⁵ cells from each of four mice were pooled and used for analysis. The results are presented as percentage of blood neutrophils from four-replicate experiments and the error bars represent the SD in the right panel.