Fbw7 controls genes that correlate with HSC quiescence and self-renewal. (A) Efficient silencing of Fbw7 expression in LSK cells and MPs in response to polyI-polyC treatment (at 2 wk after treatment). (B) Quantitative RT-PCR validation of genes selected from the microarray using mRNA from a different sorting experiment. The plotted expression values represent the relative mRNA level of each sample normalized to the median expression across all samples. (C) Quantitative RT-PCR analysis of the expression of multiple cell cycle–related genes in control (Mx-cre⁺Fbw7^+/+) and CKO (Mx-cre⁺Fbw7^fl/fl) LSK cells.