Effects of Fbw7 deletion on target protein stabilization in the BM. (A) Western blot detecting expression of Notch1-IC, uncleaved membrane Notch1 (Notch1-TM), c-Myc, cyclin E, and b-actin in total BM extracts. (B) Expression of c-Myc in cytokine-stimulated BM progenitors. Lineage⁻IL-7Rα⁻Sca-1^highc-kit^high cells from control and CKO (Mx-cre⁺Fbw7^flox/flox) mice were cultured for 48 h in the presence of cytokines. c-Myc expression was quantified using densitometry and actin normalization. Phospho–c-Myc (T58/S62) and actin levels are also shown. A representative of three experiments is shown. (C) Quantitative RT-PCR quantifying expression of Fbw7 and c-Myc in the cells used in B. Expression is normalized using b-actin.