Figure 4. Loss of Fbw7 leads to a cell-autonomous defect of HSC self-renewal. (A) 0.5 × 106 WT CD45.1 BM cells were injected into lethally irradiated WT Ly 5.1+ host mice (n = 3 for each experiment) with the same number of either control CD45.2 or CKO (Mx1-cre+Fbw7f/f) CD45.2 BM cells. The mice were analyzed 5 wk later for reconstitution of the BM, thymus, and spleen by staining cells from each tissue for both CD45.1 and CD45.2. The percent contribution of each donor to those organs is shown. CD45.2-gated cells from recipient mice were analyzed by FACS for expression of CD4, CD8, CD44, and CD25 in the thymus (D and E), B220/IgM (C), and LSK markers (B) in the BM. The cells in E are gated on the thymic Lin− population. Numbers indicate the percentage of cells in each gate.
Figure 4.

Loss of Fbw7 leads to a cell-autonomous defect of HSC self-renewal. (A) 0.5 × 106 WT CD45.1 BM cells were injected into lethally irradiated WT Ly 5.1+ host mice (n = 3 for each experiment) with the same number of either control CD45.2 or CKO (Mx1-cre+Fbw7f/f) CD45.2 BM cells. The mice were analyzed 5 wk later for reconstitution of the BM, thymus, and spleen by staining cells from each tissue for both CD45.1 and CD45.2. The percent contribution of each donor to those organs is shown. CD45.2-gated cells from recipient mice were analyzed by FACS for expression of CD4, CD8, CD44, and CD25 in the thymus (D and E), B220/IgM (C), and LSK markers (B) in the BM. The cells in E are gated on the thymic Lin− population. Numbers indicate the percentage of cells in each gate.

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