Figure 3. Loss of Fbw7 severely impairs early T cell development. (A) Absolute numbers of thymocytes (left, 2 wk after polyI-polyC; right, 4 wk after polyI-polyC) were counted from control (n = 8) and CKO (n = 8) mice and analyzed by FACS for CD4 and CD8 expression (B). (C) Absolute numbers of CD4−8− DN cells (2 wk after polyI-polyC). (D) DN cells were separated into DN1–4 populations by staining for CD44 and CD25. (E) Absolute numbers of DN2 (CD44+/CD25+) and DN3 (CD44−/CD25+) (2 wk after polyI-polyC). (F) DN thymocytes were analyzed as in D in control (Fbw7f/f, LckCre−) and CKO (Fbw7f/f, LckCre+) mice. Error bars show the SD. Numbers indicate the percentage of cells in each gate. **, P < 0.005. n = 6. (G) Absolute numbers of ETP (Lin−CD25−CD44+c-kit+) cells 2 and 4 wk after polyI-polyC injection. *, P < 0.05. n = 4.
Figure 3.

Loss of Fbw7 severely impairs early T cell development. (A) Absolute numbers of thymocytes (left, 2 wk after polyI-polyC; right, 4 wk after polyI-polyC) were counted from control (n = 8) and CKO (n = 8) mice and analyzed by FACS for CD4 and CD8 expression (B). (C) Absolute numbers of CD4−8− DN cells (2 wk after polyI-polyC). (D) DN cells were separated into DN1–4 populations by staining for CD44 and CD25. (E) Absolute numbers of DN2 (CD44+/CD25+) and DN3 (CD44−/CD25+) (2 wk after polyI-polyC). (F) DN thymocytes were analyzed as in D in control (Fbw7f/f, LckCre−) and CKO (Fbw7f/f, LckCre+) mice. Error bars show the SD. Numbers indicate the percentage of cells in each gate. **, P < 0.005. n = 6. (G) Absolute numbers of ETP (Lin−CD25−CD44+c-kit+) cells 2 and 4 wk after polyI-polyC injection. *, P < 0.05. n = 4.

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