Figure 3.
Figure 3. Connections within domains of the DRC and between the DRC and other axonemal structures. (A–G) A tomographic slice (A) and surface-rendering visualizations (B–G) of averaged 96-nm repeats of WT (C) and pWT (A, B, and D–G) are shown. A total of 10 connections are shown from different orientations. The connections are colored in magenta (isosurface and numbers 1–10). The DRC is colored yellow in WT (C) and gold in pWT (A, B, and D–G). (A–C) The DRC is the only structure in addition to the dynein arms that forms a continuous connection to the B-tubule (Bt) of the neighboring MTD (connections 1a and 1b), demonstrating that the DRC linker is the nexin link. (A and B) Similar bottom views of the DRC, but shown as tomographic slice (A) and surface-rendering representation (B). The position of the dynein stalks (arrowheads) and the IC–LC complex of the I1 dynein are indicated. (C) The cross-sectional view of WT shows that the DRC connects neighboring MTDs, whereas the uncharacterized protrusion (light green) distal of the DRC does not reach the neighboring B-tubule. (D–G) Surface renderings of the DRC and its connections to various axonemal structures from different viewpoints: distal (D), proximal (E), bottom (F), and top (G). Note that to allow better views of the DRC, different axonemal structures surrounding the DRC have been made transparent (D and E) or removed (F and G). Summary of connections: 1a and 1b, proximal and distal lobe (pL and dL) of the nexin link to neighboring B-tubule; 2, base plate to B11 structure; 3, OID link to ODA; 4, linker to tail of IA4; 5, L1 protrusion to the IA5 dynein head; 6 and 7, internal connections within the DRC linking the proximal and distal lobes (6) and the distal lobe and the L1 protrusion (7); 8, base plate to RS base; 9, DRC linker to an uncharacterized density that connects to the distal end of the IC–LC complex of I1 dynein; 10, DRC linker to an uncharacterized structure distal of the DRC. The light green protrusion shows an uncharacterized structure that was previously proposed as candidate for the nexin link (Bui et al., 2008). At, A-tubule; BP, base plate protrusion; L1 and L2, linker protrusions 1 and 2. Bar, 20 nm.

Connections within domains of the DRC and between the DRC and other axonemal structures. (A–G) A tomographic slice (A) and surface-rendering visualizations (B–G) of averaged 96-nm repeats of WT (C) and pWT (A, B, and D–G) are shown. A total of 10 connections are shown from different orientations. The connections are colored in magenta (isosurface and numbers 1–10). The DRC is colored yellow in WT (C) and gold in pWT (A, B, and D–G). (A–C) The DRC is the only structure in addition to the dynein arms that forms a continuous connection to the B-tubule (Bt) of the neighboring MTD (connections 1a and 1b), demonstrating that the DRC linker is the nexin link. (A and B) Similar bottom views of the DRC, but shown as tomographic slice (A) and surface-rendering representation (B). The position of the dynein stalks (arrowheads) and the IC–LC complex of the I1 dynein are indicated. (C) The cross-sectional view of WT shows that the DRC connects neighboring MTDs, whereas the uncharacterized protrusion (light green) distal of the DRC does not reach the neighboring B-tubule. (D–G) Surface renderings of the DRC and its connections to various axonemal structures from different viewpoints: distal (D), proximal (E), bottom (F), and top (G). Note that to allow better views of the DRC, different axonemal structures surrounding the DRC have been made transparent (D and E) or removed (F and G). Summary of connections: 1a and 1b, proximal and distal lobe (pL and dL) of the nexin link to neighboring B-tubule; 2, base plate to B11 structure; 3, OID link to ODA; 4, linker to tail of IA4; 5, L1 protrusion to the IA5 dynein head; 6 and 7, internal connections within the DRC linking the proximal and distal lobes (6) and the distal lobe and the L1 protrusion (7); 8, base plate to RS base; 9, DRC linker to an uncharacterized density that connects to the distal end of the IC–LC complex of I1 dynein; 10, DRC linker to an uncharacterized structure distal of the DRC. The light green protrusion shows an uncharacterized structure that was previously proposed as candidate for the nexin link (Bui et al., 2008). At, A-tubule; BP, base plate protrusion; L1 and L2, linker protrusions 1 and 2. Bar, 20 nm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal