Adoptive transfer of IKKβ-targeted TAMs inhibits tumor growth in vivo. (A) Representative bioluminescence imaging in vivo 14 d after adoptive transfer of TAMs isolated from established ID8 tumors (n = 6; three independent experiments). ID8-Luc were injected i.p. into syngeneic mice and tumors were allowed to develop. After 35 d, mock- and IKKβ^DN-infected TAMs (TAM_mock, TAM_DN, respectively) were adoptively transferred and peritoneal cells were collected after an additional 7 and 14 d. Bioluminescence is presented as a pseudocolor scale: red, the highest photon flux; blue, the lowest photon flux. (B) Quantification of bioluminescence from primary tumors (n = 6 each) obtained on days 0, 7, and 14. Adoptive transfer of TAM_DN significantly reduced ID8 growth on day 14 (P < 0.05; t test with Welch's correction). (C) NO secretion into the peritoneal cavity. Transfer of TAM_DN significantly increased NO production 4 h after adoptive transfer (P < 0.05; t test with Welch's correction). Data are represented as mean ± SD of n = 6. Representative data are shown from at least two independent experiments. (D) Cytokine profile in tumor ascites after 14 d. Ascitic fluid after transfer of TAM_DN contained significantly lower amounts of IL-10 and TNF-α but higher amounts of IL-12 (P < 0.01; Mann-Whitney test). Total RNA was isolated from CD11b⁺-selected TAMs for real-time PCR analysis of IL-12p40 and arginase-1 expression. Data are represented as fold induction of mRNA expression compared with TAM_mock-treated mice (n = 6). (E) MHC class II expression in the ascitic CD11b⁺ TAMs measured by FACS (percentage of positive cells indicated). Representative histograms are shown from n = 6.