F igure 5. Reconstruction of... | Rockefeller University Press

Figure 5.

$Figure 5. Reconstruction of t-BuBHQ-induced [Ca2+]i transients and their modification by caffeine and ryanodine. Simulated effects of sudden inhibition of ER Ca2+ uptake on ci (∼[Ca2+]i, A), JRelease (B), and intraluminal Ca2+ concentration, cER (∼\batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} $\mathrm{{\Delta}}\left[\mathrm{Ca}^{2\mathrm{+}}\right]_{\mathrm{ER}}^{\left(\mathrm{i}\right)}\left(\mathrm{t}\right)$ \end{document}). Simulations were performed as described in appendix B using experimentally determined descriptions of κi from Fig. 1 D, JSERCA from Fig. 2 C, and PER(vi/vERκER) from Fig. 4 D. Jpm was described by smooth curves obtained from analysis of individual cells from Fig. 4 as in Fig. 3 C, with initial values of ci based on estimates of resting [Ca2+]i in those same cells. Insets in A and B show simulations performed assuming a fixed initial value (50 nM) for ci.$

Reconstruction of t-BuBHQ-induced [Ca²⁺]_i transients and their modification by caffeine and ryanodine. Simulated effects of sudden inhibition of ER Ca²⁺ uptake on c_i (∼[Ca²⁺]_i, A), J_Release (B), and intraluminal Ca²⁺ concentration, c_ER (∼

$\mathrm{{\Delta}}\left[\mathrm{Ca}^{2\mathrm{+}}\right]_{\mathrm{ER}}^{\left(\mathrm{i}\right)}\left(\mathrm{t}\right)$

⁠). Simulations were performed as described in appendix B using experimentally determined descriptions of κ_i from Fig. 1 D, J_SERCA from Fig. 2 C, and P_ER(v_i/v_ERκ_ER) from Fig. 4 D. J_pm was described by smooth curves obtained from analysis of individual cells from Fig. 4 as in Fig. 3 C, with initial values of c_i based on estimates of resting [Ca²⁺]_i in those same cells. Insets in A and B show simulations performed assuming a fixed initial value (50 nM) for c_i.

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