fwd is required for midzone accumulation of PI4P and sGFP. (A and B) Phase–contrast (phase) and corresponding fluorescence micrographs of live preparations of dividing wild-type and fwd mutant (fwd/Df) spermatocytes coexpressing sGFP and RFP-PH-FAPP (PI4P). Colocalization of sGFP (green) and RFP-PH-FAPP (magenta) appears white (overlay). (Arrows) Midzones of late telophase cells that have been squashed (B) or in which the cleavage furrow has regressed (fwd/Df) (A). Bars, 20 µm. (A) sGFP and PI4P colocalize at the midzone in wild type (wt; top panels), but not in fwd mutant (middle and bottom panels). (B) In squashed wild-type cells, sGFP and PI4P colocalize at the midzone (top panels). The few sGFP-positive puncta present at the midzone in fwd mutant cells (bottom panels) fail to colocalize with RFP-PH-FAPP. (C) Golgi proteins localize to the poles of dividing fwd mutant cells. Fluorescence micrographs of wild-type and fwd mutant cells expressing Fws-GFP (left panels) or stained for Lva (right panels). Distribution of Fws-GFP or Lva relative to nuclei (red) is shown (overlay). (Arrows) Midzones of representative dividing cells. Bar, 20 µm.