PI4P-containing organelles localize at the midzone during cytokinesis. Phase–contrast (phase) and corresponding fluorescence time-lapse images of a dividing spermatocyte expressing RFP-PH-FAPP as a marker for PI4P and sGFP as a secretory marker. Fluorescence micrographs are shown as inverted images for clarity. Times are in min:sec. Arrowheads (t = 00:00) indicate parafusorial membranes. Arrows indicate the midzone. Colocalization of sGFP (green) and PI4P (magenta) appears white (overlay). Bar, 10 µm.