Treatment with 3MA inhibits presentation of citrullinated peptides by DCs or PECs. (A and B) Presentation of HEL to Granny (left panel labeled 48-62Cit61) or 3A9 (right panel labeled 48–62) with and without 10 mM 3MA to DCs (A) or PECs (B). (C and D) Presentation of the synthetic peptide with and without 3MA to DCs (C) or PECs (D). (E–H) Presentation of 30 µM HEL or 3 µM of the synthetic peptide to DCs (E and G) or PECs (F and H) examining different concentrations of 3MA. (I and J) Presentation of citrullinated peptide was examined after a 1-h culture in Krebs-Ringer bicarbonate buffer (KRB) after a short 2-h pulse with HEL in DCs (I) or PECs (J). Data are representative of two to three experiments. Error bars indicate SEM.