Figure 3. Proangiogenic effects of DHT in male ECs is VEGF dependent. (A) Production of VEGF by male ECs exposed to DHT for 48 h. Total cell lysate protein was quantified and assayed via ELISA (n = 4 independent experiments). P = 0.002 using ANOVA for linear trend. (B) Expression of Flt-1 (P = 0.0128) and (C) KDR (P = 0.0295) mRNA and (D) Flt-1 and (E) KDR (P = 0.0005) protein in male ECs exposed to DHT for 48 h (using ANOVA for linear trend). (F) Vascular network formation in male ECs treated with DHT with and without 1 µg/ml anti-VEGF antibody or 10 µM of the PI3K inhibitor LY294002 (n = 3 independent experiments for B–F, respectively). ***, P < 0.001 compared with control using ANOVA. All data are expressed as means ± SEM. For each independent experiment, cells from a different donor were used.
Figure 3.

Proangiogenic effects of DHT in male ECs is VEGF dependent. (A) Production of VEGF by male ECs exposed to DHT for 48 h. Total cell lysate protein was quantified and assayed via ELISA (n = 4 independent experiments). P = 0.002 using ANOVA for linear trend. (B) Expression of Flt-1 (P = 0.0128) and (C) KDR (P = 0.0295) mRNA and (D) Flt-1 and (E) KDR (P = 0.0005) protein in male ECs exposed to DHT for 48 h (using ANOVA for linear trend). (F) Vascular network formation in male ECs treated with DHT with and without 1 µg/ml anti-VEGF antibody or 10 µM of the PI3K inhibitor LY294002 (n = 3 independent experiments for B–F, respectively). ***, P < 0.001 compared with control using ANOVA. All data are expressed as means ± SEM. For each independent experiment, cells from a different donor were used.

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