Comparison of the phenotypes of WT, ABIN1[D485N], ABIN1[D485N]/MyD88^−/−, and MyD88^−/− mice. (A) Spleen sizes of WT, ABIN1[D485N]xMyD88^+/+, ABIN1[D485N]xMyD88^−/−, and MyD88^−/− mice. Bar, 1 cm. A typical result from many mice analyzed is shown. (B) Flow cytometric analysis was performed as in Fig 2, and a representative set of contour plots is presented (top) showing the proportion of germinal center (GC) B cells (B220⁺ GL-7⁺ CD95⁺) in WT, ABIN1[D485N]xMyD88^+/+, ABIN1[D485N]xMyD88^−/−, and MyD88^−/− mice. Similar results were obtained in three independent experiments. On the bottom, the percentage of GC B cells is shown for each WT type (filled squares), ABIN1[D485N]xMyD88^+/+ (filled triangles), ABIN1[D485N]xMyD88^−/− (open triangles), and MyD88^−/− (open squares) mouse examined. Each symbol represents an individual mouse and the horizontal bar shows the mean of the values obtained. (C) Anti-dsDNA antibodies and ANA antibodies in ABIN1[D485N]xMyD88^+/+ mice (filled squares) and ABIN1[D485N]xMyD88^−/− mice (filled triangles). Data are shown for a single experiment with six to eight mice of each genotype. ***, P < 0.005; **, P > 0.005 (Mann-Whitney U test). Each symbol represents an individual mouse and the horizontal bar shows the mean of the values obtained. (D) Hematoxylin and eosin (H&E) staining (first two panels) and PAS staining (third and fourth panels) of kidney sections from ABIN1[D485N]xMyD88^+/+ and ABIN1[D485N]xMyD88^−/−. Bars, 0.05 mm. The arrow shows PAS-positive material. Three or four mice of each genotype were analyzed and a representative result is shown.