Autoimmune phenotypes in ABIN1[D485N] mice. Values were compared by the Mann-Whitney U test. *, P ≤ 0.05; **, P ≥ 0.005; ***, P ≤ 0.005. (A) Serum levels of immunoglobulin isotypes quantitated by ELISA in WT and ABIN1[D485N] mice. Error bars represent mean ± SEM. (B) Level of anti-dsDNA antibodies in serum of 16-wk-old WT, ABIN1[D485N], and heterozygous (HET) mice measured by ELISA. (C) Total antinuclear antibodies (ANA; left) and anti-dsDNA antibodies (right) in the serum of 22-wk-old WT and ABIN1[D485N] mice were measured by ELISA. (B and C) Each symbol represents one mouse and the horizontal bars show the mean of the values obtained. (D) Kidney sections stained with fluorescein isothiocyanate–labeled anti–mouse C3, C1q, and IgG. (E) Kidney sections stained with hematoxylin and eosin (top) or PAS (bottom). Bar, 0.05 mm. In the top panel, the arrowhead shows glomerulonephritis with capillary loop thickening by eosinophilic material and the black arrow shows infiltration of mesangium by neutrophils. In the bottom panel, the arrow shows glomerulonephritis, with capillary wall thickening by PAS-positive staining. (F–I) Hematoxylin and eosin–stained sections of spleen, heart, lungs, and liver. (F) Spleen. The arrow indicates the WT (left) or congested splenic artery in ABIN1[D485N] mice (right) by eosinophilic material. Bars, 0.1 mm. (G) Heart. The arrow indicates infiltration of neutrophils and macrophages around arterial wall (A) in ABIN1[D485N] mice. M, myocardium. Bars, 0.2 mm. (H) Lungs. The arrows show plasma cells in the WT and ABIN1[D485N] mice. B, bronchus. Bars, 0.1 mm. (I) Liver, the portal tracts are shown by arrows. Bars, 0.1 mm. The data are from a single experiment with 7–9 (A–C) or 6–12 (D–I) mice per genotype.