TB chemotherapy prolongs survival of Card9^−/− mice. (A) Survival of mice infected with 400 CFUs MTB H37Rv and treated with INH/RIF (AB) at 3 wk p.i. Lung bacterial burdens (B) and serum G-CSF levels (C) at AB begin. (D) Survival of mice infected with 400 CFUs MTB H37Rv and treated with AB at 2 wk p.i. (E) MTB burdens in lungs and spleens of WT and KO mice before and after AB treatment. Data from one (A–C; n = 15) or two experiments (D and E; n = 15). Mann-Whitney test was applied for statistical analyses, for survival Kaplan-Meier curves were used. *, P < 0.05; ***, P < 0.001.