Susceptibility of Card9^−/− mice to tuberculosis resides in the hematopoietic compartment and is linked to Card9^−/− PMN deficiencies to produce both pro- and antiinflammatory cytokines during TB infection. (A) BM chimeras were infected with 200 CFUs of MTB H37Rv and survival was followed up for 65 d p.i. (n = 8–10). (B) CARD9 expression in myeloid cells and AECs. (C) PMN from WT and Card9^−/− mice were infected with MTB H37Rv at MOI 5 and bacterial multiplication assed by [³H]Uracil incorporation. TNF (D) and IL-10 (E) levels in supernatants of MTB-infected PMN, determined by ELISA (MOI 5). Data are mean ± SEM of two experiments with six replicates each (C) or are representative of four independent experiments with three to six replicates each (D and E). *, P < 0.05; **, P < 0.01; ***, P < 0.001, Student's t test.