The adaptor molecule CARD9 converges signals from multiple PRRs involved in MTB recognition. (A) Deficient inflammatory cytokine production by Card9^−/− BMDMs in response to heat-killed MTB or after blocking of phagocytosis. (B) Blocking dectin-1–syk pathway in WT cells recapitulated the observations reported for Card9^−/− BMDMs, as revealed by selective impairment of TNF and IL-6 production. (C) mAGP induced diminished cytokine levels in Card9^−/− macrophages. Data are mean ± SEM of two independent experiments with three replicates each. *, P < 0.05; **, P < 0.01; ***, P < 0.001, Student's t test.