Card9^−/− BMDMs control MTB despite impaired cytokine synthesis. (A) BMDMs from WT and Card9^−/− mice were infected with MTB H37Rv at MOI 5 and bacterial multiplication was assed by [³H]Uracil incorporation. (B) WT and Card9^−/− BMDMs were infected with gfp-BCG at different MOI and mycobacterial uptake was monitored by FACS analysis of the F4/80⁺ population, insert lower MOI (0.4 and 2) were assessed as well. (C) Card9^−/− BMDMs were not hampered in NO release, NO in cell culture supernatants was assessed by Griess reaction. (D) Cell death after MTB infection was evaluated by nucleosome enrichment in the post-nuclear fraction using ELISA. (E) Cytokines in supernatants of infected cell were determined by ELISA. Data are mean ± SEM of two experiments with six replicates each (A) or are representative of three independent experiments with three replicates each (B–E). **, P < 0.01; ***, P < 0.001, Student's t test.