Figure 1. Card9−/− mice fail to control pulmonary tuberculosis. (A) Survival of WT and Card9−/− mice (n = 15) after aerosol infection with virulent MTB H37Rv (450 CFUs), Kaplan-Meier curves, and log-rank test. (B) Bacterial burdens in lungs and spleens of infected animals (n = 5). Data from one of four experiments, Mann-Whitney test for statistic analysis. (C) Ziehl-Neelsen staining of acid fast bacilli in lungs of infected mice. (D–F) Histopathology of lungs; Giemsa staining (D) of paraffin-embedded tissue showed acute pneumonia with multiple necrotic foci in susceptible mice; immunostaining for iNOS did not reveal major differences (E); TUNEL staining revealed accelerated cell death in Card9−/− mice (F). TUNEL-TMred and nuclei are DAPI stained. Tissue were collected at 28 d p.i. Data in C–F are representative of two independent experiments (n = 5). Bars: (C) 20 µm; (D) 2.5 mm; (E) 200 µm; (F) 100 µm. **, P < 0.01.
Figure 1.

Card9−/− mice fail to control pulmonary tuberculosis. (A) Survival of WT and Card9−/− mice (n = 15) after aerosol infection with virulent MTB H37Rv (450 CFUs), Kaplan-Meier curves, and log-rank test. (B) Bacterial burdens in lungs and spleens of infected animals (n = 5). Data from one of four experiments, Mann-Whitney test for statistic analysis. (C) Ziehl-Neelsen staining of acid fast bacilli in lungs of infected mice. (D–F) Histopathology of lungs; Giemsa staining (D) of paraffin-embedded tissue showed acute pneumonia with multiple necrotic foci in susceptible mice; immunostaining for iNOS did not reveal major differences (E); TUNEL staining revealed accelerated cell death in Card9−/− mice (F). TUNEL-TMred and nuclei are DAPI stained. Tissue were collected at 28 d p.i. Data in C–F are representative of two independent experiments (n = 5). Bars: (C) 20 µm; (D) 2.5 mm; (E) 200 µm; (F) 100 µm. **, P < 0.01.

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