Altered development of intestinal CD3⁻NKp46⁺ cells in Il7^−/− mice. (A) NK1.1 versus CD127 expression on gated CD3⁻NKp46⁺ splenocytes and small intestinal (SI) LPLs of WT versus Il7^−/− mice. Subset frequencies are indicated in one representative experiment out of six performed. (B) Absolute numbers of total CD3⁻NKp46⁺ cells and CD127/NK1.1 subsets from small intestinal LPLs in WT and Il7^−/− mice (n = 6 mice of each genotype analyzed). Significant differences are indicated. Values are presented as means ± SD. (C) RORγt expression in the indicated intestinal CD3⁻NKp46⁺ subsets in WT (bold line) and Il7^−/− mice (shaded histogram). The dotted line depicts staining of CD3⁻NKp46⁺ cells from Rorc^−/− mice. (D) IL-22 production from intestinal CD3⁻NKp46⁺ subsets in WT and Il7^−/− mice. Results indicate frequencies from one out of three independent experiments.