Normal development of splenic and intestinal CD3⁻NKp46⁺ cell subsets requires Id2. (A) NK1.1 versus CD127 expression on gated CD3⁻NKp46⁺ splenocytes and small intestinal (SI) LPLs of WT and Id2^−/− mice. Subset frequencies are indicated in one representative experiment out of six performed. (B) RORγt expression in intestinal CD3⁻NKp46⁺ subsets in WT (bold line) and Id2^−/− mice (shaded histogram). The dotted line depicts staining of CD3⁻NKp46⁺ cells from Rorc^−/− mice. (C) IL-23–induced IL-22 expression in intestinal CD3⁻NKp46⁺CD127⁺ cells from WT and Id2^−/− mice. Results indicate frequencies from one out of two independent experiments.