Figure 3. A20 deficiency sensitizes IECs to DSS-induced apoptosis. (A) TUNEL staining on distal colon sections of A20IEC-KO mice and control littermates (WT) after 2 and 6 d of 1.5% DSS treatment, and after 9 d during recovery (6 d of 1.5% DSS followed by normal drinking water). Bars, 150 µm. (B) Quantification of the number of TUNEL-positive cells/field from untreated and DSS-treated mice. Error bars represent SEM. *, P < 0.05. (C) Detection of BrdU incorporation on distal colon sections of A20IEC-KO mice and control littermates (WT) after 6 d of 1.5% DSS treatment, and after 9 d during recovery (6 d of 1.5% DSS followed by normal drinking water). Bars: (day 4) 50 µm; (day 9) 250 µm. (D) Quantification of the number of BrdU-positive cells/field from untreated and DSS-treated mice. Error bars represent SEM. Data are representative of three independent experiments.
Figure 3.

A20 deficiency sensitizes IECs to DSS-induced apoptosis. (A) TUNEL staining on distal colon sections of A20IEC-KO mice and control littermates (WT) after 2 and 6 d of 1.5% DSS treatment, and after 9 d during recovery (6 d of 1.5% DSS followed by normal drinking water). Bars, 150 µm. (B) Quantification of the number of TUNEL-positive cells/field from untreated and DSS-treated mice. Error bars represent SEM. *, P < 0.05. (C) Detection of BrdU incorporation on distal colon sections of A20IEC-KO mice and control littermates (WT) after 6 d of 1.5% DSS treatment, and after 9 d during recovery (6 d of 1.5% DSS followed by normal drinking water). Bars: (day 4) 50 µm; (day 9) 250 µm. (D) Quantification of the number of BrdU-positive cells/field from untreated and DSS-treated mice. Error bars represent SEM. Data are representative of three independent experiments.

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